Nucleotide sequence for yeast dihydrolipoamide dehydrogenase.

Dihydrolipoamide dehydrogenase from halophilic archaebacteria.

Dihydrolipoamide dehydrogenase has been discovered in the halophilic archaebacteria for the first time. The enzyme from both classical and alkaliphilic halobacteria has been investigated. (1) The enzyme specifically catalysed the stoichiometric oxidation of dihydrolipoamide by NAD+. Enzymic activity was optimal at 2 M-NaCl and was remarkably resistant to thermal denaturation. (2) The relative m...

Nucleotide sequence of yeast GDH1 encoding nicotinamide adenine dinucleotide phosphate-dependent glutamate dehydrogenase.

The yeast GDH1 gene encodes NADP-dependent glutamate dehydrogenase. This gene was isolated by complementation of an Escherichia coli glutamate auxotroph. NADP-dependent glutamate dehydrogenase was overproduced 6-10-fold in Saccharomyces cerevisiae bearing GDH1 on a multicopy plasmid. The nucleotide sequence of the 1362-base pair coding region and 5' and 3' flanking sequences were determined. Tr...

Dihydrolipoamide dehydrogenase: a 'new' function for an old enzyme?

Nucleotide sequence of the yeast SUC2 gene for invertase.

The yeast SUC2 gene is a structural gene for both the secreted and intracellular forms of invertase. We have determined the nucleotide sequence of the coding region and the 5' and 3' flanking regions. The coding regions for the signal peptide-containing precursor to secreted invertase and for the intracellular invertase begin at different initiation codons within the SUC2 gene but share the sam...

Nucleotide sequence of a white clover alcohol dehydrogenase cDNA.

We have isolated a cDNA clone for white clover (Jrifolium REFERENCES repensL. cv Huia) alcohol dehydrogenase 1 (ADH1, EC. from a root cDNA library using a partial pea ADH1 cDNA (1) (1987) / Mol BM 195 115-123 as a probe. This clone encodes a polypeptide of 380 amino acids.